Part:BBa_I714926:Design

RecA_DlexO_DLacO3

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

NOR gate

wild type recA promoter, with two lexA binding sites and two lacI binding site. The lexA binding site is operator of recA promoter, the lacI binding site is lacO3. One of the lexA binding sites is at the same location as wild type recA promoter and the other one is 30bp upstream the wild type one. The two lacO3 sites locate at the same positions as in wild type lac promoter.

Source

synthetic